Department of Zoology

Name: Dr Romana Sarwar
Designation: Assistant Professor
Department: Department of Zoology
Faculty: Biosciences
Qualification: PhD Biosciences
Area of Specialization: Cancer Biology and Molecular Genetics
Contact Number -
Email Address: romzsarwar@gmail.com
Honors/Awards/Medals:
  • International Research Support Initiative Program in PhD (Fully funded), UK
  • Indigenous Scholarship from Higher Education Pakistan in PhD (Fully funded), Pak        
  • Doctoral Program Coordination Committee Scholarship for research aid in M. Phil, Pak
  • Merit scholarship in M. Sc.
  • Merit scholarship in M. Phil on getting top position in Punjab University, Pak
  • Position holder in M. Phil and M. Sc
  • Stood first in Matric examinations and distinctions in 4 subjects awarded.
Research Interest and Courses teaching:
  • Research interest is in, Molecular Genetics, Cancer Genetics, Molecular Biology, DNA Repair pathways and Mutagenesis, Cell Culture and Gene Knock down/out models. Lab Skills includes, cell culturing, cloning, genetic engineering/modification (CRISPR/Cas9 technology), knockdown model using RNAi (siRNA) approach, DNA extraction, RNA extraction by manual and kit method, Genotyping techniques using ARMS PCR, RFLP, Expressional Analysis using Real Time PCR and Immunohistochemistry Techniques. Courses Teaching: Biochemistry, Biotechnology, Research methodology, Bioinformatics, Advances in analytical techniques
Projects:
“Studies on Genetic Characterization and Deregulation of Homologous Recombinant Repair (HRR) Pathway Genes in Thyroid Cancer”.
Publications:
  • Sadaf, S., Rasheed, M., Sarwar, R., & Akhtar, S. K. M. W. (2015) Molecular cloning, sequence characterization and expression analysis of somatotropin gene of an indigenous chicken breed (Aseel). Pak. J. Biochem. Mol. Biol, 48(1), 7-14.
  • Sarwar R., Bashir, K., Saeed, S., Mahjabeen, I., & Kayani, M. A. (2016) Association of Promoter Polymorphisms in Xrcc2 Gene Involved in DNA Double Strand Break Repair and Increased Susceptibility to Thyroid Cancer Risk in Pakistani Population. Journal of Carcinogenesis & Mutagenesis, 7: 265 
  • Sarwar R., Mahjabeen I., Bashir K., Saeed S., & Kayani, M. A. (2017). Haplotype Based Analysis of XRCC3 Gene Polymorphisms in Thyroid Cancer. Cellular Physiology and Biochemistry, 42(1), 22-33.    
  • Sarwar, R., Sheikh, A. K., Mahjabeen, I., Bashir, K., Saeed, S., & Kayani, M. A. (2017). Upregulation of RAD51 expression is associated with progression of thyroid carcinoma. Experimental and Molecular Pathology, 102(3), 446-453.
  • Saeed, S., Mahjabeen, I., Sarwar, R., Bashir, K. and Kayani, M.A. (2017) Haplotype analysis of XRCC2 gene polymorphisms and association with increased risk of head and neck cancer. Scientific Reports, 7(1), 13210-13220.
  • Bashir, K., Sarwar, R., Fatima, S., Saeed, S., Mahjabeen, I., & Akhtar, K. M. (2018). Haplotype analysis of XRCC1 gene polymorphisms and the risk of thyroid carcinoma. Journal of BUON.: official journal of the Balkan Union of Oncology, 23(1), 234-243.
  • Bashir, K., Sarwar R., Saeed, S., Mahjabeen, I., & Kayani, M. A.  2018 Interaction among susceptibility genotypes of PARP1 SNPs in Thyroid carcinoma PLOS one, 13(9), 1-12   
Brief portfolio:
I did my MSc Biology from Quaid-e-Azam University, Islamabad and MPhil Biochemistry from University of the Punjab, Lahore. Completed PhD in Biosciences (May 2018) from COMSATS University, Islamabad, Pakistan. My research in Cancer Genetics and Epigenetics was focused on the role of DNA repair genes in DNA damage and thyroid cancer progression and identified the association of many carcinogens in thyroid cancer. I voluntarily worked in many cancer hospitals where not only I collected blood samples and tumor samples for my research but also learnt biochemical techniques in their molecular biology labs. I successfully awarded scholarship for six months fellowship in University of Leicester, UK. There, I started work focused on uncovering the roles of Rad51 paralogs in DNA repair and genome stability maintenance. Despite having only a short amount of time, I established new cell lines and cell culture approaches by CRISPR/Cas9 mediated genome editing protocols, mutation detection assays and generated mutant XRCC2 and XRCC3 cell lines for future studies. Also used various cell culture approaches involving RNAi /siRNA mediated knockdown assays